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From MS/MS spectra to PSTs
 

The Taggor module is part of the PepLine software pipeline for proteomic analysis. It has been developed in the context of the Helix CEA/LCP collaboration.

Taggor aims at determining a set of PST candidates from a processed MS/MS spectrum (i.e. a peak-list) without complete peptide reconstruction or protein database query.

The Taggor algorithm involves three steps:
-  All sequences of N amino acids (N equals 3 to 5) are generated.
-  Each of these sequences is aligned to the MS/MS spectrum according to the Y ions reading orientation. If a sequence matches a set of peaks, then flanking masses are computed and a new PST is generated. A score is defined for each PST: it consists in the product of the relative intensities of spectrum peaks that correspond to the PST anchoring sequence.
-  Finally, a set of best PST candidates (usually 20 PSTs) is retained for further analysis.

Taggor software overview - 17.5 ko
Taggor software overview

 
In the same section
From MS/MS spectra to PSTs
From PSTs to protein identification
From PSTs to gene localization
Validation of PepLine
 
On the same subject
CEA/LCP, Grenoble
From MS/MS spectra to PSTs
From PSTs to protein identification
PepLine: high throughput proteomics
From PSTs to gene localization
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