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From PSTs to protein identification
 

The PSTs, which have been automatically generated from MS/MS spectra analysis, are submitted to a second software component: PepMap. PepMap is responsible of mapping the PSTs with the contents of protein databases (or, directly, to complete translated chromosomes).

PepMap algorithm consists in two steps:
-  Mapping a PST on a polypeptide sequence consists in searching for the PST sequence part, then checking that at least one of the flanking masses corresponds to the adjacent sequences.

Mapping of a PST on a protein sequence - 4.3 ko
Mapping of a PST on a protein sequence

-  Protein identification results in extracting proteins on which the more PST matches occur.

PepMap protein identification overview - 9.2 ko
PepMap protein identification overview

 
In the same section
From MS/MS spectra to PSTs
From PSTs to protein identification
From PSTs to gene localization
Validation of PepLine
 
On the same subject
CEA/LCP, Grenoble
From MS/MS spectra to PSTs
From PSTs to protein identification
PepLine: high throughput proteomics
From PSTs to gene localization
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