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Peptide Sequence Tag (PST)

Peptide Sequence Tags (PSTs) (Mann94) can be easily generated from MS/MS spectra analysis. A PST is defined as a short peptide sequence (3 to 5 amino acids) flanked by two masses corresponding to the two adjacent polypeptides.

Peptide Sequence Tag - 15.8 ko
Peptide Sequence Tag

These two masses give information on the two unknown sub-sequences corresponding to the spectrum areas that are not easily interpretable. Using PSTs as results of mass spectrometry analysis offers several advantages:

-  Only few peaks (n+1 where n is the size of the PST sequence) are needed for the PST determination that is therefore more easy to obtain than the complete peptide sequence (especially when the spectrum is noisy).

-  Generating several overlapping PSTs from one spectrum may contribute to produce de-novo-sequencing-like results while avoiding the frequent misinterpretations due to de-novo algorithms.

-  From the algorithmic point of view, rapid pattern-matching of short PST sequences can be achieved very efficiently since the small size of PST sequences allow to use hash-coding techniques.

In the same section
Mass spectometry (MS vs MS/MS)
Peptide Sequence Tag (PST)
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